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Physical, Chemical and Biological Treatment of Rice Husk to Improve Its Nutrative Value

by Rahat Naseer (2003-VA-196) | Dr. Abu Saeed Hashmi | Dr. Muhammad Tayyab | Prof. Dr. Habib ur Rehman.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: Thesis submitted without CD. Availability: Items available for loan: UVAS Library [Call number: 2450-T] (1).

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Molecular Phylogeny And Diversity Analysis Of Bovidae (Boselaphus Tragocamelus, Antilope Cervicapra) And Cervidae (Axis Axis, Axis Porcinus) In Pakistan

by Ghulam Abbas (2011-VA-748) | Dr. Asif Nadeem | Prof. Dr. Mansoor Ellahi Babar | Dr. Muhammad Tayyab.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Many species of mammals have declined within the past two centuries due to human caused disturbances and the unsustainable use of natural resources. Molecular methods have an important role in phylogeny and diversity analysis. The present study was designed for diversity analysis of Boselaphus tragocamelus & Antelope cervicapra (Bovidae) and Axis axis & Axis porcinus (Cervidae) family in Pakistan. A total of 25 samples from each of the four species were collected from different parks, zoos and natural habitats. DNA was extracted, PCR primers were designed and cytochrome-b, cytochrome-c gene and d-loop regions were amplified by PCR. PCR products were sequenced bi-directionally by Big DyeTM Terminator. Bioinformatics tools, Blast 2 sequences, Clustal-W, MEGA-6, Bioconductor in “R” were applied for analysis. The clustering of the samples indicates that each species contains less within-population genetic variability. Same pattern was observed when sequence of three genes was combined and MDS plot was constructed. Phylogenetic analysis of the gene sequences revealed that each species comprised a clade that is clearly distinct from the clade comprised of other species of deer selected for this study. Finding of this study indicated that these species of deer have significant genetic variations among-species that differentiate them from each other. This is the first report from our region. The information of selected species of deer is prerequisite for designing effective strategy in future conservation practices. However further genomic investigations should be carried out at larger scale. Availability: Items available for loan: UVAS Library [Call number: 2560-T] (1).

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